Staff Research Associate - 133037

Under supervision, will independently perform research techniques, and troubleshoot new and established assay techniques. Independently perform and analyze in vivo and in vitro experiments to study the role of liver damage in development of liver cancer. Responsible for culturing and maintaining cells in vitro, stimulating with agonists and reagents, isolating RNA and proteins, analyzing RNA expression by QPCR and protein expression by western blot. Assist lab personnel and coordinate laboratory support. Directly train and provide work direction for the daily activities of undergraduate assistants and/or junior technical staff. Orient new lab members, i.e. visiting researchers, post-doc and students on lab policies. Provide research coordination and ensure laboratory safety, maintain laboratory inventory, perform general lab maintenance and order supplies. The goal of this project is to understand how liver damage due to obesity and NASH can lead to liver cancer. Other duties assigned as needed.

• Theoretical knowledge of immunology, molecular biology, cell biology, and protein chemistry.

• Demonstrated experience performing standard molecular biology techniques, including PCR, SDS Page electrophoresis, western blotting, ELISA assays, and recombinant DNA manipulation.

• Knowledge of various cell based assays pertaining to investigating cell phenotypes.

• Demonstrated experience performing culture primary cells and immortalized cells in vitro. Knowledge of immune cell types.

• Ability to follow UCSD Safety and Hazardous Materials guidelines. Ability and willingness to work with hazardous/biohazardous materials.

• Excellent organizational skills to order and maintain lab equipment and order supplies. Ability to maintain confidentiality.

• Demonstrated experience performing and willingness to handle blood, plasma and other tissue specimens for processing and shipping. Must be willing and able to work with animals, biohazardous materials and radioactivity.

• Demonstrated experience performing mice tail vein bleeding for various studies.

• Demonstrated experience performing various mice handling techniques.

• Demonstrated experience establishing rodent obesity models including high fat feeding and lipid infusion.

• Demonstrated knowledge and experience in flow cytometry and operation of a FACS calibur instrument. Ability performing and interpreting results from experiments with human and mouse cells stained with specific fluorochrome-conjugated monoclonal antibodies.

• Demonstrated experience in maintaining well-organized lab and thorough records. Basic math skills and ability to handle accounting and billing practices.

• Ability to work under supervision and independently on projects with a variety of personalities.

• Employment is subject to a criminal background check.

• Must be willing and able to work weekends and evenings when necessary.

• Must be willing to work with mice.

• Must be trained in and able to handle hazardous/biohazardous materials.

If employed by the University of California, you will be required to comply with our Policy on Vaccination Programs, which may be amended or revised from time to time. Federal, state, or local public health directives may impose additional requirements.

If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable.

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

To foster the best possible working and learning environment, UC San Diego strives to cultivate a rich and diverse environment, inclusive and supportive of all students, faculty, staff and visitors. For more information, please visit UC San Diego Principles of Community.

UC San Diego is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.

For the University of California's Affirmative Action Policy please visit: https://policy.ucop.edu/doc/4010393/PPSM-20
For the University of California's Anti-Discrimination Policy, please visit: https://policy.ucop.edu/doc/1001004/Anti-Discrimination

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

UC San Diego Health maintains a marijuana and drug free environment. Employees may be subject to drug screening.

Misconduct Disclosure Requirement: As a condition of employment, the final candidate who accepts a conditional offer of employment will be required to disclose if they have been subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct; received notice of any allegations or are currently the subject of any administrative or disciplinary proceedings involving misconduct; have left a position after receiving notice of allegations or while under investigation in an administrative or disciplinary proceeding involving misconduct; or have filed an appeal of a finding of misconduct with a previous employer.

a. "Misconduct" means any violation of the policies or laws governing conduct at the applicant's previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, discrimination, dishonesty, or unethical conduct, as defined by the employer. For reference, below are UC's policies addressing some forms of misconduct:

• UC Sexual Violence and Sexual Harassment Policy
• UC Anti-Discrimination Policy
• Abusive Conduct in the Workplace