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JOB SUMMARY The Staff Research Associate (SRA) III will participate in the investigation of the cardiopulmonary health effects of inhalation of tobacco smoke and other air pollutants. The SRA III will be under direct supervision of Dr. Mehrdad Arjomandi. The SRA III will assist with collection and processing of human biological samples including blood, urine, sputum, bronchoalveolar lavage, and resected lung tissue samples for further molecular and functional characterization of the immune and non-immune cells using a variety of ex-vivo and in-vitro assays such as flow and mass cytometry, genomics, and functional assays. In addition, the SRA III will assist with maintaining and organize a cryo-biorepository of human biological samples including live-frozen primary human cells in liquid nitrogen and human plasma and BAL samples in ultra-cold freezers. The SRA III will also assist in maintaining accurate records for the biorepository as well as the associated clinical data from the participating human subjects. The SRA III will also assist with recruitment, screening, coordination of the visits of the human subject participants who donate biological samples. The SRA III may also assist in performing tests such as pulmonary function testing, skin allergy testing, questionnaire administration and vital signs measurement. Furthermore, the SRA III will maintain accurate data records, enter data, process, store, and ship biological samples, perform lab-based assays on human biological samples, assist in maintaining and monitoring all equipment and procedures for quality control, assist in trouble shooting discrepancies, ordering and stocking lab supplies and perform other duties as assigned. This is a critical position requiring fingerprinting and background check prior to hire, as the incumbent will have direct access to controlled substances or hazardous materials and direct access to and is responsible for protected, personal and other sensitive data. DUTIES & ESSENTIAL JOB FUNCTIONS Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job. The functions should add up to 100%.
% of time |
Essential Function (Yes/No) |
Key Responsibilities (To be completed by Supervisor) |
25 |
Y |
Collection and maintenance of a repository for clinical transplant samples. This will involve attending some clinical procedures, consenting patients, and processing of blood and lung tissue samples for storage. Accurate records of enrolled subjects and obtained samples must be maintained. This project requires teamwork and will at times be performed during off hours, due to the nature of clinical research. |
50 |
Y |
Collection and processing of human biological samples including blood, urine, sputum, bronchoalveolar lavage, and resected lung tissue samples. Performing molecular and functional characterization of the immune and non-immune cells using ex-vivo and in-vitro assays involving tissue culture and multi-parameter flow and mass cytometry. Perform immunohistochemistry, Elisa, Western blotting and other biochemical lab techniques. Analyze and discuss the data with research supervisors and collaborators. |
10 |
Y |
Lab management: Handle lab ordering and work with suppliers. |
5 |
Y |
Clinical research: Abstraction and maintenance of medical records corresponding with the collected samples. Participation in the conduct, analysis, and write-up of retrospective and/or prospective research projects under direct supervision of the investigators. |
10 |
Y |
Learn new techniques and protocols related to the translational projects including experiments and assays to determine the state of immune activation. |
0 |
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0 |
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0 |
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0 |
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0 |
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0 |
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100% |
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(To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.) |
Qualifications, Licenses and Certifications Required Qualifications:
- BA/BS in related science and knowledge of, or experience with, the basic techniques or methods required by the position, or an equivalent combination of education and experience.
- Knowledge of general lab and cell biology/culture procedures.
- Minimum 2-3 years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position, or an equivalent combination of education and experience with cell culture techniques including sterile techniques, maintenance of cell lines, freezing and thawing of samples, in vitro cell assays such as cell survival assays and migration assays.
- Experience with general molecular biology techniques such as ELISA assay, DNA and RNA purification, PCR assay, genotyping, and western blot.
- Strong programming skills.
- Willingness to work with human blood, tissue, bronchoalveolar lavage samples, etc.
- Willingness to work with human subjects, including assisting with procedures involving human subjects, such as obtaining and processing bronchoalveolar lavage and human lung tissue from proceduralist, surgeon, or pathologist.
- Willingness to consent patients for clinical studies and to work with clinical staff to collect samples.
- Computer literate; advance database skills including knowledge of Microsoft Excel; Able to learn Microsoft Access, R/Bioconductor.
- Excellent communication skills.
- Excellent organizational skills.
- Detail oriented; works with a high level of accuracy.
- Professional and motivated attitude experience with cell biology, tissue culture, flow cytometry, molecular biology, or related experience in a laboratory setting.
Preferred Qualifications:
- Two to five years of wet laboratory experience with molecular biology procedures
- Strong proficiency in tissue culture including cell counting, sterile technique, and cell viability and growth assays
- Experience with tissue culture (tissue handling, preparation of slides and slide staining)
- Advance Understanding of statistics.
- Data analysis with Excel.
- Demonstrated critical thinking skills.
- Experience with of electronic health records.
- UCSF work experience in academic, research, teaching and clinical departments
- Experience working with the UCSF Committee on Human Research
About UCSF
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences. We bring together the world's leading experts in nearly every area of health. We are home to five Nobel laureates who have advanced the understanding of cancer, neurodegenerative diseases, aging and stem cells.
Pride Values
UCSF is a diverse community made of people with many skills and talents. We seek candidates whose work experience or community service has prepared them to contribute to our commitment to professionalism, respect, integrity, diversity and excellence - also known as our PRIDE values.
In addition to our PRIDE values, UCSF is committed to equity - both in how we deliver care as well as our workforce. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care. Additional information about UCSF is available here.
Join us to find a rewarding career contributing to improving healthcare worldwide.
Equal Employment Opportunity
The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.
Salary Information
The final salary and offer components are subject to additional approvals based on UC policy.
Your placement within the salary range is dependent on a number of factors including your work experience and internal equity within this position classification at UCSF. For positions that are represented by a labor union, placement within the salary range will be guided by the rules in the collective bargaining agreement.
To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html Qualifications, Licenses and Certifications Required Qualifications:
- BA/BS in related science and knowledge of, or experience with, the basic techniques or methods required by the position, or an equivalent combination of education and experience.
- Knowledge of general lab and cell biology/culture procedures.
- Minimum 2-3 years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position, or an equivalent combination of education and experience with cell culture techniques including sterile techniques, maintenance of cell lines, freezing and thawing of samples, in vitro cell assays such as cell survival assays and migration assays.
- Experience with general molecular biology techniques such as ELISA assay, DNA and RNA purification, PCR assay, genotyping, and western blot.
- Strong programming skills.
- Willingness to work with human blood, tissue, bronchoalveolar lavage samples, etc.
- Willingness to work with human subjects, including assisting with procedures involving human subjects, such as obtaining and processing bronchoalveolar lavage and human lung tissue from proceduralist, surgeon, or pathologist.
- Willingness to consent patients for clinical studies and to work with clinical staff to collect samples.
- Computer literate; advance database skills including knowledge of Microsoft Excel; Able to learn Microsoft Access, R/Bioconductor.
- Excellent communication skills.
- Excellent organizational skills.
- Detail oriented; works with a high level of accuracy.
- Professional and motivated attitude experience with cell biology, tissue culture, flow cytometry, molecular biology, or related experience in a laboratory setting.
Preferred Qualifications:
- Two to five years of wet laboratory experience with molecular biology procedures
- Strong proficiency in tissue culture including cell counting, sterile technique, and cell viability and growth assays
- Experience with tissue culture (tissue handling, preparation of slides and slide staining)
- Advance Understanding of statistics.
- Data analysis with Excel.
- Demonstrated critical thinking skills.
- Experience with of electronic health records.
- UCSF work experience in academic, research, teaching and clinical departments
- Experience working with the UCSF Committee on Human Research
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